The phage display based selection of pathogen antigens allows the identification of immunogenic proteins which cannot be discovered by classical methods like 2D-PAGE.Here, we demonstrate a powerful selection system for novel immunogenic antigens based on the display of protein fragments from genomic libraries of pathogens on M13 filamentous phage.Furthermore, this technology also includes a unique selection of open reading frames (ORF) during phage production to enhance the quality of the library, the selection and screening process.Examples for the application of this technology will be given (e.g.new immunogenic antigens of Mycoplasma hyopneumniae and M.mycoides).Furthermore, we developed a complete pipeline for the generation of human antibodies against the identified new immunogenic proteins.