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Background To investigate the influence of dark zona pellucida on the oocyte and embryo quality, fertilization, implantation and pregnancy outcome.Methods A total of 42 patients retrieving oocytes with dark zona pellucida (DZP) in the IVF/ICSI cycles were collected as the study group, and 102 infertile patients of whose oocytes were surrounded with normal zona pellucida (NZP) were received as the control group.The patients in the study group were subdivided into two groups, including the partial oocytes with DZP group and allthe oocytes with DZP group.