Objective: To evaluate the prognostic significance of hTERC gene amplification in patients with cervical intraepithelial neoplasia grade i (CIN1).Methods: A total of 2,499 women aged 30 years to 49 years were screened in a population-based cervical cancer screening study in the rural areas of Jiangxi province.A total of 74 CIN1 patients were originally diagnosed by pathological morphological evaluation.The patients were then diagnosed clinically and pathologically using hybrid capture-2 (HC-2) to identify the high-risk human papillomavirus (hr-HPV) and fluorescence in situ hybridization to determine the hTERC gene amplification baseline.All patients were followed up with informed consent.At the first follow-up, hr-HPV HC-2 was performed 12 months after screening.The second follow-up was performed 24months after screening using hr-HPV HC-2, colposcopy, and pathological morphological evaluation using either conization or four quadrant biopsy colposcopically.