[目的]将猪繁殖与呼吸综合征病毒(PRRSV) GP5基因重组到大肠杆菌和乳酸菌中,对GP5蛋白进行原核表达,为在肠道中定居存活且传递GP5蛋白抗原提供基础。[方法]从云南PRRSV病料中提取RNA、进行RT-PCR,获得约835bp的GP5基因片段。设计合成带有Nco Ⅰ和Xba Ⅰ酶切位点的一对扩增缺失信号肽的GP5基因(dGP5)表达引物、进行PCR,获得约513bp的目的片段。双酶切后分别
The H9N2 avian influenza virus (AIV) has become increasingly concerning due to its role in severe economic losses in the poultry industry.Transmission of AIV to mammals,including pigs and humans,has a
The avian sarcoma and leukosis virus (ASLV) family of retroviruses contains six highly related subgroups,A to E and J,thought to have evolved from a common viral ancestor in chicken.The susceptibility
The aim was to identify codon usage bias between the newly comfirmed Salmonella pullorum outer membrane protein C (ompC) gene (GenBank accession No.CP003047) and that of other 21 reference Enterobacte
This study was designed to evaluate the toxicological effect of dietary nickel chloride (NiCl2) on the counts of intestinal bacteria and diversity of microorganisms in broilers.Plate counting and poly
In this study,we attenuated a Chinese QX-like nephropathogenic infectious bronchitis virus (IBV) strain.YX10,by passaging through fertilized chicken eggs.The 90th passage strain (YX10p90) was selected
To investigate the effects of photosynthetic bacteria as additives on water quality,microbial community structure and diversity,a photosynthetic purple non-sulfur bacteria,Rhodopseudamonas palustris,w
The increasing prevalence of obesity worldwide is associated with a parallel increase in non-alcoholic fatty liver disease (NAFLD).To investigate the effect of Lactobacillus johnsonii BS15 on NAFLD,12
[目的]本试验旨在探索酸性蛋白酶水解鸭血球蛋白的最优工艺条件,开发一种新型肽粉,并研究其功能特质与体外抗氧化能力.[方法]通过单因素与正交试验调查酶用量、温度、pH、水解时间等因素对酶解反应的影响,确定最优工艺参数,研究其营养、功能与抗氧化特性.[结果]酸性蛋白酶酶解鸭血球蛋白的最优工艺参数为：酶用量(E/S) 6000 U/g,温度50℃,pH3.5,水解时间7h.采用该工艺条件,鸭血球蛋白水解