An improved culture system for adult DRG neurons

来源 :The 18th Congress of the International Federation of Associa | 被引量 : 0次 | 上传用户：liongliong429

【摘要】

：

【作者】

：

Zhao J.H Zhang H.Y Dong X Zhang X.F Lao M.L Zhang Q.P Yi X.N

【机构】

：

Department of Anatomy, Hainan Medical College, Haikou 571199, China

【出处】

：

The 18th Congress of the International Federation of Associa

【发表日期】

：

2014年8期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

Dissociated adult DRG neurons offer the possibility to study mature neurons that may better resemble the in vivo characteristics of these cells.However, adult DRG neurons culture is a complex, time-consuming process.In this study,DRGs were dissected and stripped in F12 and dissociated with collagenase, trypsin and DNAse prior to mechanical trituration.Compared with traditional method, this one reduced the experimental time from 3 hrs to 110 mins.

其他文献

Horizons in development

Our discovery of rivet-like structures in the amnion that pin together key tissue layers is cause for optimism amongst medical scientists exploring causes of premature births following preterm rupture

会议

The prevalence of ankyloglossia congenital anomaly in riverine areas of south east nigerian populati

This research was done to determine the occurrence of ankyloglossia developmental anomaly in Riverine Areas of South East Nigerian Population.1640 newborn babies delivered in selected hospitals, healt

会议

Excess caffeine exposure impairs eye development during chick embryogenesis

Caffeine has been an integral component of our diet and medicines for centuries.It is now known that over-consumption of caffeine has detrimental effects on our health,and also disrupts normal fetal d

会议

Role of high mobility group Box 1 in hyperthermia-induced neural tube defects

Neural tube defects (NTDs) are a group of severe congenital malformations resulting from the failure of neurulation.Neurogenesis leads to the formation of the neural tube through neural stem cell (NSC

会议

PAR4 up-regulated expression of TRPV1 through PKA signaling pathway in cultured primary sensory neur

Protease-activated receptor 4 (PAR4) is localized in primary sensory neurons and is believed to play an important role in nociceptive response through TRPV1 sensitization.In this study, we examined th

会议

Novel expression and functional implication of Ras-related protein 1 during mouse spermatogenesis

Genes involved in proliferation and apoptosis are clearly important for the occurrence of mature spermatogenesis.Herein, we provide compelling evidence for the expression and functional role of Ras-re

会议

High frequency electromagnetic radiation effects on blood-brain-barrier of rat and the protective ef

To investigate the effect of high frequency radiation on the BBB of hippocampus of SD rat and the protective effect of the amdo lam capsule.After irradiation to observe changes in the different time p

会议

Effects of allogeneic olfactory bulb tissue and cells transplantation on expressions of substance P

To investigate the changes of allogeneic olfactory bulb tissue and cells transplantation on the levels of substance P and NF-κB in the spinal cord of rats with neuropathic pain.SD rats were randomly d

会议

Chronic constraint stress induces a different type of insulin-resistant hyperglycemia compared with

We have proposed a hypothesis that neuron impairment or loss in the brainstem is the essential step toward type 2 diabetes.According to this viewpoint, the traditional streptozotocin (STZ) rat model i

会议

Oxidative stress-induced central renin-angiotensin system overactivation is involved in hypertension

Our earlier study has reported that active vitamin D deficiency in mice causes secondary hypertension and cardiac dysfunction, but underlying mechanism is still largely unknown.To clarify whether exog

会议

An improved culture system for adult DRG neurons

与本文相关的学术论文