【摘 要】
:
A medulloblastoma cell line from pTet-on/pTRE-SV40Tag transgenic mouse, named MB39, was established in our laboratory.This cell line has been serially subcu
【机 构】
:
ShanghaiInstituteofBrainFunctionalGenomics,EastChinaNormalUniversity,Shanghai,200062,Shanghai,China
【出 处】
:
Wnt信号、器官发育和干细胞国际学术大会(Wnt Signaling,Organogenesis,and Stem Ce
论文部分内容阅读
A medulloblastoma cell line from pTet-on/pTRE-SV40Tag transgenic mouse, named MB39, was established in our laboratory.This cell line has been serially subcuitured over 100 times in 1640 medium presenting no sign of cell senescence.The MB39 cell line has a doubling time of about 19.5h.Under phase-contrast microscope, the cells are dendritic-like.lmmunocytochemical analysis of medulloblastoma markers demonstrated that cells are positive for SV40Tag and Math1.The cells can produce tumors in nude mice.Four new linear-furocoumarins 1,2,3 and 4 were found to show significant inhibition against this cell line with IC50s of 5.1, 2.8, 7.5 and 4.4μg/ml,respectively.
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