Objective: To investigate the relation of TFPI-2 gene polymorphism and acute coronary syndrome (ACS) in Chinese Han population.Methods: The polymorphism of TFPI-2 gene was detected by PCR-sequencing in all people recruited, and a case control study was carried out in three groups, the ACS group which consisted of 140 ACS patients (including 68 unstable angina patients and 72 acute myocardial infarction patients), the SA group which included 273 SA patients, and the control group which consisted of 306 healthy people.Results Eight single nucleotide polymorphism (SNP) sites were identified in TFPI-2 gene in all subjects, the allele and genotype frequencies were different on the two SNP sites: rs59805398 and rs34489123 among case and control group.Linkage disequilibrium among the SNPs of rs3763473, rs59999573, rs59740167, rs34489123 of TFPI-2 gene was found and C-C-G-G-G and T-C-G-G-G were predominant haplotypes in ACS patients.Linkage disequilibrium among the SNPs of rs59999573, rs59740167, rs34489123 of TFPI-2 gene was found and C-G-G-G and G-A-A-A were predominant haplotypes in SA patients.There was no significant difference between ACS group and SA group.Conclusions C allele in rs59805398 and A allele in rs34489123 have correlation with coronary heart disease (CHD) and they might be a higher risk factor of CHD among Chinese Han population.There is apparent linkage disequilibrium among SNPs of TFPI-2 gene in CHD patients.