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Glycerol-3-phosphate acyltransferase (GPAT) is the key enzyme responsible for the acylation of glycerol-3-phosphate to lysophosphatidic acid.GPAT9 is one member of GPAT gene family that encodes GPAT.In this study, DNAs of GPAT9 were isolated from 10 peanut cultivars by homologous cloning strategy.