【摘 要】
：
Degeneration of basal forebrain cholinergic neurons(BFCNs)is associated with cognitive impairments of Alzheimers disease(AD),implying that BFCNs hold potentials in exploring stem cell-based replacemen
【机 构】
：
State Key Laboratory of Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes
【出 处】
：
第九届海内外华人神经科学家研讨会(The 9th Symposium for Chinese Neuroscientis
论文部分内容阅读
Degeneration of basal forebrain cholinergic neurons(BFCNs)is associated with cognitive impairments of Alzheimers disease(AD),implying that BFCNs hold potentials in exploring stem cell-based replacement therapy for AD.However,studies on derivation of BFCNs from embryonic stem cells(ESCs)are limited,and the application of ESC-derived BFCNs remains to be determined.Here,we report differentiation approaches for directing both mouse and human ESCs into mature BFCNs.These ESC-derived BFCNs exhibit features similar to those of their in vivo counterparts,and acquire appropriate functional properties.After transplantation into basal forebrain of AD model mice,ESC-derived BFCN progenitors predominantly differentiate into mature cholinergic neurons that functionally integrate into endogenous basal forebrain cholinergic projection system.The AD mice grafted with mouse or human BFCNs exhibit improvements in learning and memory performances.Our findings suggest the promising perspective of ESC-derived BFCNs in development of stem cell-based therapies for treatment of AD.
其他文献
Precise timing is critical for motor control such as singing and speech.Chain networks,in which groups of neurons form feed-forward networks that support sequential activations of successive neurons,a
With the technology advancing,we are accumulating data at the molecular,cellular,tissue,behavior and environmental level.As a consequence,how to first identify(disease)roots at various levels and then
Humans and monkeys have access to an accurate representation of visual space despite a constantly moving eye.One mechanism by which the brain accomplishes this is by remapping visual receptive fields
My research goal is to develop mass spectrometry-based proteomics and systems biology approaches to address biomedical challenges.Wehave developed a quantitative proteomics pipeline,capable of analyzi
Yes-associated protein(YAP)is a key transcriptional cofactor of the Hippo pathway,critical for the development of multiple organs.However,its role in the developing brain remains poorly understood.Her
Histone variants as important epigenetic regulators play important roles in development.However,it is still unknown that how histone variant H2AZ regulates cortical neurogenesis during brain developme
Glycogen synthase kinase-3β(GSK-3β)is a key regulator of many cellular signaling pathways.In the dopamine(DA)system,GSK-3β is particularly involved in D2 receptor(D2R)-mediated signaling.We and others
Small packaging capacity has limited therapeutic use of AAV in genetic disease caused by large genes such as cysic fibrosis.A novel trans-splicing strategy was designed to overcome the size constraint
Hippocampal long-term potentiation(LTP)can be temporally and mechanistically classified into early phase LTP(E-LTP)and late phase LTP(L-LTP).While the non-decaying nature of L-LTP is thought to be dep
Non-motor symptoms are of vital importance in Parkinsons disease in recent years.These symptoms occur far early before the motor dysfunction in PD.However,the mechanisms underlying these non-motor sym