论文部分内容阅读
Spleen deficiency is a very important pathology in children asthma based on TCM theory.The intestinal bacterial flora is reported to be imbalanced in spleen deficiency.The imbalance of intestinal bacterial flora is believed to be closely related to the attack and development of asthma.Spine-pinching manipulation has good effect on strengthening the spleen and prevention and treatment of children asthma.Whether it is related to improve the imbalance ofintestinal baaerial flora in children with asthma is still unknown.
Objective：To evaluate the influence of spine-pinching manipulation on intestinal bacteria flora of asthma rats with spleen deficiency,which may help with understanding the good therapeutic efficacy of spine-pinching manipulation on children asthma.
Method：In total44rats were randomly divided into normal control group(group C,n=8),asthma group(group A n=12),asthma with spleen deficiency group(group SA n=12)and asthma with spleen deficiency with spine-pinching group(group PSA n=12).The general state and body weight of the rats were observed periodically.The rectal temperature of rats was taken after collecting feces at several time points.The feces was collected before the first time of OVA sensitisation and just after asthma being induced.The feces was stored in an ice box,and smeared for gram staining within4hours at the same day.The ratio of bacillus to the total bacteria count(BW).the ratio of positive bacillus to the total bacteria count(B)and the ratio of positive cocci to the total bacteria count(C)were calculated under the microscope for the evaluation of the balance of intestinal bacteria flora.All the rats in group C.six rats selected randomly from group A,SA and PSA were sacrificed on the next day after the induction of asthma.Then the right lung tissues were collected and prepared for HE staining for pathology evaluation.All data were presented as means±SEM.SPSS18.0and GraphPad Prism6.00were used for statistical analysis and figures respectively.The comparison of change in weight and anal temperature among all the groups were analyzed by repeated measurement analysis of variance(ANOVA).Data in different groups of each time point were analyzed pair wisely by multivariate ANOVA.Data of non-repeated measure were analyzed by one-way ANOVA for multiple comparisons among groups.Data between two groups were analyzed by Fishers Least Significant Difference(LSD)test for homogeneity in variance and Dunnets T3for heterogeneity in variance.A P value less than0.05was considered as significant.
Result：Before OVA sensitisation,the weight gain in group SA and PSA was significantly slower than group C(P＜0.01,P＜0.01).The rectal temperature in group SA and PSA was significantly higher than group C before OVA sensitisation(P＜0.01,P＜0.01).At week6and7before OVA sensitisation,the weight gain in group PSA was significantly more than group SA(P＜0.05),but the rectal temperature in group PSA was not significantly different from group SA.After asthma induction the weight gain in group A,SA and PSA was significantly less than group C(P＜0.01.P＜0.01,P＜0.01).The weight gain in group SA and PSA was still significantly less than group A(P＜0.01,P＜0.01).The weight gain in group PSA was significantly more than group SA(P＜0.01).The rectal temperature in group A was higher than group C but not significantly(P＞0.05).The rectal temperature in group SA and PSA was significantly higher than group C(P＜0.01.P＜0.01)and that in group PSA was significantly lower than in group SA(P＜0.05).Before OVA sensitisation,BW in group SA and PSA was significantly lower than group C(P＜0.01,P＜0.01).But BW in group PSA was statistically higher than group SA(P＜0.05).B in group SA and PSA was lower than group C significantly(P＜0.01,P＜0.01).But B in group PSA was higher than group SA statistically(P＜0.01).C in group SA and PSA washigher than group C significantly(P＜0.01,P＜0.01).But C in group PSA was lower than group SA statistically(P＜0.05).After asthma induction via OVA aerosol challenge,BW in group A.SA and PSA was statistically less than group C(P＜0.05,P＜0.01,P＜0.01).B in group A.SA and PSA was statistically less than group C(P＜0.01,P＜0.01,P＜0.01).C in group A.SA and PSA was statistically less than group C(P＜0.05,P＜0.01,P＜0.01).BW and B in group PSA was higher statistically than group SA(P＜0.01,P＜0.01).C in group PSA was lower than group SA statistically(P＜0.01).Based on HE staining,pathology in group A,SA and PSA showed obvious inflammation.Among those groups,the severity of group SA and PSA was more than group A and group PSA was less than group SA.
Objective：To evaluate the influence of spine-pinching manipulation on intestinal bacteria flora of asthma rats with spleen deficiency,which may help with understanding the good therapeutic efficacy of spine-pinching manipulation on children asthma.
Method：In total44rats were randomly divided into normal control group(group C,n=8),asthma group(group A n=12),asthma with spleen deficiency group(group SA n=12)and asthma with spleen deficiency with spine-pinching group(group PSA n=12).The general state and body weight of the rats were observed periodically.The rectal temperature of rats was taken after collecting feces at several time points.The feces was collected before the first time of OVA sensitisation and just after asthma being induced.The feces was stored in an ice box,and smeared for gram staining within4hours at the same day.The ratio of bacillus to the total bacteria count(BW).the ratio of positive bacillus to the total bacteria count(B)and the ratio of positive cocci to the total bacteria count(C)were calculated under the microscope for the evaluation of the balance of intestinal bacteria flora.All the rats in group C.six rats selected randomly from group A,SA and PSA were sacrificed on the next day after the induction of asthma.Then the right lung tissues were collected and prepared for HE staining for pathology evaluation.All data were presented as means±SEM.SPSS18.0and GraphPad Prism6.00were used for statistical analysis and figures respectively.The comparison of change in weight and anal temperature among all the groups were analyzed by repeated measurement analysis of variance(ANOVA).Data in different groups of each time point were analyzed pair wisely by multivariate ANOVA.Data of non-repeated measure were analyzed by one-way ANOVA for multiple comparisons among groups.Data between two groups were analyzed by Fishers Least Significant Difference(LSD)test for homogeneity in variance and Dunnets T3for heterogeneity in variance.A P value less than0.05was considered as significant.
Result：Before OVA sensitisation,the weight gain in group SA and PSA was significantly slower than group C(P＜0.01,P＜0.01).The rectal temperature in group SA and PSA was significantly higher than group C before OVA sensitisation(P＜0.01,P＜0.01).At week6and7before OVA sensitisation,the weight gain in group PSA was significantly more than group SA(P＜0.05),but the rectal temperature in group PSA was not significantly different from group SA.After asthma induction the weight gain in group A,SA and PSA was significantly less than group C(P＜0.01.P＜0.01,P＜0.01).The weight gain in group SA and PSA was still significantly less than group A(P＜0.01,P＜0.01).The weight gain in group PSA was significantly more than group SA(P＜0.01).The rectal temperature in group A was higher than group C but not significantly(P＞0.05).The rectal temperature in group SA and PSA was significantly higher than group C(P＜0.01.P＜0.01)and that in group PSA was significantly lower than in group SA(P＜0.05).Before OVA sensitisation,BW in group SA and PSA was significantly lower than group C(P＜0.01,P＜0.01).But BW in group PSA was statistically higher than group SA(P＜0.05).B in group SA and PSA was lower than group C significantly(P＜0.01,P＜0.01).But B in group PSA was higher than group SA statistically(P＜0.01).C in group SA and PSA washigher than group C significantly(P＜0.01,P＜0.01).But C in group PSA was lower than group SA statistically(P＜0.05).After asthma induction via OVA aerosol challenge,BW in group A.SA and PSA was statistically less than group C(P＜0.05,P＜0.01,P＜0.01).B in group A.SA and PSA was statistically less than group C(P＜0.01,P＜0.01,P＜0.01).C in group A.SA and PSA was statistically less than group C(P＜0.05,P＜0.01,P＜0.01).BW and B in group PSA was higher statistically than group SA(P＜0.01,P＜0.01).C in group PSA was lower than group SA statistically(P＜0.01).Based on HE staining,pathology in group A,SA and PSA showed obvious inflammation.Among those groups,the severity of group SA and PSA was more than group A and group PSA was less than group SA.