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被囊内动物是以单体或群体生活的海栖动物,本文将就近二年来在完成国家重点课题海洋药物研究中——海洋生物活性物质的提取分离与纯化,及其抗肿瘤活性的部分研究工作进行报告.经过对所获得的被囊类动物属原料(HQS)按照规定的提取工艺,首先得到HOS总提取物,经进一步采用薄层色谱(TLC),柱层析色谱(CC),反相高效液相色谱(RP-HPLL)等方法分离纯化获得了HQS提取物Ⅰ(HQSⅠ)与提取物Ⅱ(HQSⅡ)和提取物Ⅲ(HQSⅢ),经化学法和紫外光谱法初步鉴定,在上述各部位HQS提物中分别含有多肽与
The tunicate animal is a marine animal that lives in a singleton or group. This article will be completed in the last two years in the completion of the national key research marine drug research - the extraction, separation and purification of marine biological active substances, and part of their anti-tumor activity research. The work is reported. After the obtained extraction process of the tunicate raw material (HQS) according to the regulations, the total extract of HOS is firstly obtained, and further chromatography is performed by thin layer chromatography (TLC) and column chromatography (CC). The HQS extract I (HQSI) and extract II (HQSII) and extract III (HQSIII) were isolated and purified by phase-to-phase high-performance liquid chromatography (RP-HPLL) and were initially identified by chemical and ultraviolet spectroscopy. Each part of the HQS extract contains polypeptides and