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本文研究了不同培养温度对鼠-鼠杂交瘤HB-32细胞的生长、单克隆抗体(McAb)产生、葡萄糖消耗和乳酸产生的影响。 作者用含有10％胎牛血清,葡萄糖(4g/L),NaHCO_3(2.2g/L)草酰乙酸(13.2mg/L),胰岛素(0.8mg/L).丙酮酸钠(5.5mg/L),非必需氨基酸和1％抗真菌抗生素DMEM培育HB-32细胞。 作者发现,①最大活细胞浓度(Xvmax)依赖于培养温度。37℃细胞浓度为3.2×10~6/ml,与已报道的同一细胞系结果相似;33℃
This study investigated the effects of different culture temperatures on the growth of mouse-mouse hybridoma HB-32 cells, the production of monoclonal antibodies (McAbs), glucose consumption, and lactic acid production. The authors used 10% fetal bovine serum, glucose (4g/L), NaHCO3 (2.2g/L) oxaloacetate (13.2mg/L), insulin (0.8mg/L). sodium pyruvate (5.5mg/L) HB-32 cells were incubated with DMEM, a non-essential amino acid and 1% antifungal antibiotic. The authors found that 1 the maximum viable cell concentration (Xvmax) depends on the culture temperature. The cell concentration at 37°C was 3.2×10~6/ml, which was similar to the same reported cell line; 33°C