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目的:研究人ING4基因对乳腺癌细胞MCF-7的生长抑制作用。方法:通过实时荧光定量-PCR(real-time fluorogen-tic quantitative-PCR,RFQ-PCR)检测3种乳腺癌细胞株中ING4 mRNA的表达水平;ING4基因的表达质粒转染MCF-7细胞;MTT法和FCM法检测ING4基因对MCF-7细胞增殖的影响;Annexin-Ⅴ/PI双染法观察细胞凋亡情况;RT-PCR法分析转染ING4后p53、p21及bax基因的转录表达情况。结果:在3种乳腺癌细胞MCF-7、MDA-MB-435和MDA-MB-231中ING4的表达均明显低于正常乳腺组织。转染ING4表达载体的MCF-7细胞较对照组细胞生长速度减慢(P<0.05);细胞周期中G1期比例增加,S期比例减少;细胞凋亡率升高。p53的表达未见明显变化,而p21和bax表达显著上调。结论:ING4与乳腺癌的发生发展具有一定相关性;高表达ING4基因能够抑制人乳腺癌细胞MCF-7的生长。
AIM: To investigate the inhibitory effect of human ING4 gene on the growth of breast cancer cell line MCF-7. Methods: The expression of ING4 mRNA in three breast cancer cell lines was detected by real-time fluorogen-tic quantitative-PCR (RFQ-PCR). The expression of ING4 gene was transfected into MCF-7 cells. MTT The effect of ING4 on the proliferation of MCF-7 cells was detected by FCM and FCM. The apoptosis of MCF-7 cells was detected by Annexin-V / PI double staining. The transcriptional expression of p53, p21 and bax genes were detected by RT-PCR. Results: The expression of ING4 in breast cancer cells MCF-7, MDA-MB-435 and MDA-MB-231 was significantly lower than that in normal breast tissues. The growth of MCF-7 cells transfected with ING4 expression vector was slower than that of control group (P <0.05). The proportion of cells in G1 phase increased and the proportion of S phase decreased. The apoptosis rate of MCF-7 cells increased. No significant changes in the expression of p53, p21 and bax expression was significantly up-regulated. CONCLUSION: ING4 is associated with the occurrence and development of breast cancer. High expression of ING4 can inhibit the growth of human breast cancer cell line MCF-7.