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目的探讨SARS冠状病毒(SARS-CoV)的起源。方法运用生物信息学的方法,比较不同SARS样冠状病毒之间的异同,着重分析蝙蝠所携带的SARS样冠状病毒(SL-CoV)跨越种属屏障传播的可能性。结果除编码棘突蛋白(S蛋白)基因外,SARS-CoV与蝙蝠SL-CoV间编码结构蛋白的基因与编码复制酶的基因相似程度均很高;而S基因的差异主要集巾在S1片段,且该片段的GC含量与SARS-CoV基因组的GC含量明显不同。进一步分析表明,由S1片段构建的冠状病毒进化树与由S2片段构建的进化树存在拓扑结构上的不平行性。结论人类SARS病毒可能来源于蝙蝠SL-CoV,但在S基因内发生了基因重组事件,从而导致其获得跨越种属屏障传播的能力。
Objective To investigate the origin of SARS-CoV. Methods The bioinformatics method was used to compare the similarities and differences between different SARS-like coronaviruses. The possibility of SARS-like coronavirus (SL-CoV) carried by bats crossing the barrier of species was emphasized. Results The gene encoding the structural protein between SARS-CoV and bat SL-CoV was highly similar to the gene encoding replicase, except for the S-protein coding gene. The difference of S gene was mainly located in the S1 fragment , And the GC content of this fragment was significantly different from that of SARS-CoV genome. Further analysis showed that there was a topological structural non-parallel between the coronavirus phylogenetic tree constructed by S1 fragment and the phylogenetic tree constructed by S2 fragment. Conclusion The human SARS virus may originate from the bat SL-CoV, but a gene recombination event occurs in the S gene, resulting in its ability to spread across the species barrier.