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目的:研究稳定斑块组织因子(TF)活化表达及在血栓形成中的作用。方法:建立ApoE-/-小鼠动脉粥样硬化病变的动物模型,一期凝固法检测小鼠血浆TF特异性的促凝活性,光镜和电镜观察稳定斑块病变,免疫组化SP法检测小鼠稳定斑块TF的蛋白表达,原位杂交法检测小鼠稳定斑块TF mRNA表达。结果:ApoE-/-小鼠血浆促凝活性显著增加(P<0.01),并随着诱发斑块时间的延长呈上升趋势,抗TF单抗能显著抑制血浆促凝活性(P<0.01);实验小鼠的病变为稳定斑块,电镜下,ApoE-/-小鼠可见完整的血管内皮细胞,在内皮完整的血管内有血小板的聚集;不同狭窄程度的ApoE-/-小鼠动脉粥样硬化病变部位TF的表达差异有统计学意义(P<0.01),TF的表达随着粥样硬化病变的进展而增强,TFmRNA与TF蛋白表达一致。结论:TF可能通过活化和释放启动凝血过程,导致血栓的形成;TF不仅是粥样硬化导致动脉损伤的结果,还可能是促进动脉粥样硬化病变的重要原因。
Objective: To study the activated expression of stable plaque tissue factor (TF) and its role in thrombosis. Methods: Animal models of atherosclerotic lesions in ApoE - / - mice were established. The coagulation assay was used to detect the specific procoagulant activity of TF in mice. Stable plaque lesions were observed under light microscope and electron microscope. Mouse stable plaque TF protein expression, in situ hybridization assay mouse stable plaque TF mRNA expression. Results: The plasma procoagulant activity of ApoE - / - mice increased significantly (P <0.01), and increased with the prolongation of plaque time. Anti - TF mAb could significantly inhibit the procoagulant activity of plasma (P <0.01). The lesions of experimental mice were stable plaques. Under electron microscopy, ApoE - / - mice showed intact vascular endothelial cells with platelet aggregation in intact endothelium. ApoE - / - mice with different degree of stenosis had atherosclerosis The expression of TF in cirrhosis was significantly different (P <0.01). The expression of TF increased with the progression of atherosclerosis, and the expression of TF mRNA was consistent with that of TF. Conclusion: TF may initiate the process of coagulation through activation and release, leading to the formation of thrombus. TF is not only the result of atherosclerosis leading to arterial injury, but also may be an important cause of atherosclerotic lesions.