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目的探讨光学相干层析成像技术(optical coherence tomography,OCT)判断大鼠动脉球囊损伤后再狭窄程度的可行性。方法将60只雄性SD大鼠随机分成对照组(假手术组30只)和实验组(球囊损伤30只),每个时间点(造模前、造模后2、7、14和28 d)取6只,制备大鼠颈总动脉球囊导管扩张损伤模型,在各个时间点将动脉组织行OCT检测并和血清学指标(LDL、HDL、ALP、CRP)、病理学指标(血管内膜与中膜厚度比值、管腔狭窄率)相比较。结果实验组血清学指标(LDL/HDL、ALP)及病理学指标都随着造模时间逐渐加重,对照组处理前后无明显变化,两组相比差别具有显著意义(P<0.05)。实验组OCT图象散射系数(μs)随造模时间逐渐增大,对照组μs处理前后无明显变化,两组相比差别具有显著意义(P<0.05);实验组μs与病理学指标(血管内膜与中膜厚度比值、管腔狭窄率)均存在高度相关性(P<0.01)。结论 OCT是评估动脉球囊损伤后再狭窄程度的可行方法。
Objective To investigate the feasibility of using optical coherence tomography (OCT) to determine the degree of restenosis after arterial balloon injury in rats. Methods Sixty male Sprague-Dawley rats were randomly divided into control group (30 in sham operation group) and experimental group (30 in balloon injury group). At each time point (before model making, 2, 7, 14 and 28 d ) Were used to prepare the common carotid artery balloon catheter in rats. The arterial tissues were examined by OCT at various time points and compared with the serum markers (LDL, HDL, ALP, CRP), pathology Compared with the ratio of media thickness, lumen stenosis rate). Results Serum parameters (LDL / HDL, ALP) and pathological indexes of the experimental group increased gradually with the time of modeling, but there was no significant change in the control group before and after treatment. There was significant difference between the two groups (P <0.05). The scattering coefficient (μs) of OCT image in the experimental group increased with the modeling time, but there was no significant difference between the two groups (P <0.05). The μs of the experimental group and the pathological parameters Intima-media thickness ratio and lumen stenosis rate) were highly correlated (P <0.01). Conclusion OCT is a feasible method to assess the degree of restenosis after arterial balloon injury.