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利用生理剂量碘作用于早期人胎大脑神经细胞无血清培养物,经离子交换法分离出神经细胞的神经原特异性烯醇化酶(NSE)和非神经原特异性烯醇化酶(NNE)。两酶的转换方式与体内相似,碘促进神经细胞 NNE(αα,αγ亚基)向 NSE(γγ亚基)转换。培养25天 KI(?)组NSE 含量比培养30天对照组含量高,培养30天 KI_2组 NSE 含量是对照组的3倍,且 NSE 出现提前,活性增高。培养15天时加碘的作用比开始培养时加碘的作用更加明显。
Serum iodine (NSE) and non-neuron specific enolase (NNE) were isolated from neurons by ion-exchange method using physiological dose of iodine on serum-free culture of neurons in early human fetal brain. The conversion of the two enzymes is similar to that in vivo, and iodine promotes the conversion of NNE (αα, αγ subunit) to NSE (γγ subunit) in nerve cells. The content of NSE in KI (?) Group cultured for 25 days was higher than that in control group for 30 days. The content of NSE in KI2 group was 3 times higher than that in control group after 30 days of culture, and the NSE content was earlier and the activity was higher. The effect of iodine when cultured for 15 days is more pronounced than that for iodine when starting culture.