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目的探讨替米沙坦对糖尿病大鼠胰岛硫氧还蛋白(TRX)与硫氧还蛋白相互作用蛋白(TXNIP)表达的影响。方法 SD大鼠分为正常对照(NC)、糖尿病(DM)和替米沙坦干预(TM)组。DM组和TM组大鼠高脂高糖喂养4周后,腹腔注射链脲佐菌素(STZ)(30mg/kg)构建2型糖尿病(T2DM)模型,TM组予替米沙坦10mg·kg~(-1)·d~(-1)干预,8周后测定血浆生化指标、胰岛中胰岛素、TRX和TXNIP的表达。结果 DM组和TM组空腹血糖、丙二醛以及胰岛中TXNIP水平均较NC组高(P<0.05),TM组血糖与DM组差异无统计学意义,但TM组上述其余指标水平较DM组低(P<0.05);DM组和TM组空腹胰岛素、过氧化氢酶、超氧化物歧化酶及胰岛中胰岛素和TRX水平低于NC组(P<0.05),但TM组高于DM组(P<0.05)。结论 T2DM大鼠胰岛TRX表达较正常大鼠降低,而TX-NIP升高;氧化应激水平和低胰岛素血症的改善可能与替米沙坦干预了这种变化有关。
Objective To investigate the effect of telmisartan on the expression of TRX and TXNIP in diabetic rats. Methods SD rats were divided into normal control (NC), diabetes mellitus (DM) and telmisartan intervention (TM) groups. Rats in DM group and TM group were fed with high-fat and high-sucrose for 4 weeks, and type 2 diabetes (T2DM) was induced by intraperitoneal injection of streptozotocin (STZ) (30mg / kg) ~ (-1) · d ~ (-1) intervention. After 8 weeks, the serum biochemical indexes, insulin, TRX and TXNIP expression in islets were measured. Results The levels of fasting blood glucose, malondialdehyde and TXNIP in pancreatic islets in DM group and TM group were significantly higher than those in NC group (P <0.05). There was no significant difference in TM group between DM group and DM group (P <0.05). The levels of fasting insulin, catalase, superoxide dismutase and pancreatic insulin and TRX in DM group and TM group were lower than those in NC group (P <0.05), but higher in TM group than in DM group P <0.05). Conclusions The expression of TRX in islets of T2DM rats is lower than that in normal rats, while TX-NIP is increased. The improvement of oxidative stress and hypoproteinemia may be related to the intervention of telmisartan.