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目的 :用显微注射法制备携带EB病毒 (Epstein Barrvirus,EBV)膜抗原 (membraneantigen ,MA)基因 BLLF1的转基因小鼠。方法 :对 38只昆明种小鼠进行超排卵 ,收集受精卵 ,将EBVMA基因 BLLF1显微注射到受精卵的原核内。将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育直至分娩。PCR分析检测仔鼠基因组中转基因的整合。结果 :显微注射 6 77个受精卵 ,其中成活且健康的 32 0个 ,卵的成活率为 47.2 %。将其移植到 12只假孕母鼠的输卵管内 ,其中 2只鼠怀孕并产仔 12只 ,出生率为 3 .75 %。PCR分析 5只仔鼠基因组整合有BLLF1基因 ,整合率为 41.7%。结论 :携带EBVMA基因 BLLF1的转基因小鼠已制备成功
OBJECTIVE: To prepare transgenic mice bearing Epstein Barr virus (EBV) BLLF1 gene by microinjection. Methods: Thirty-eight Kunming mice were subjected to superovulation and the fertilized eggs were harvested. The EBVMA gene BLLF1 was microinjected into the pronucleus of fertilized eggs. After injection, a viable and healthy fertilized egg is transplanted into the fallopian tube of a pseudopregnant female mouse to develop until delivery. PCR analysis detects the integration of transgenes in the offspring genome. RESULTS: 6 77 fertilized eggs were microinjected, of which 32 were alive and healthy, with a 47.2% survival rate. Transplanted into the fallopian tubes of 12 fake pregnant rats, 2 of them were pregnant and 12 were born, with a birth rate of 3.75%. PCR analysis of 5 offspring genome integration BLLF1 gene, the integration rate was 41.7%. Conclusion: The transgenic mice carrying EBVMA gene BLLF1 have been successfully prepared