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目的探讨反义ｃ－ｍｙｃ基因质粒对鼠脑胶质瘤细胞Ｃ６增殖及凋亡的作用。方法将可在真核细 胞表达大鼠反义 ｃ－ｍｙｃ基因的质粒 ｐＣＥＰ４ＡＳＣＭＲ以脂质体 ＦｕＧＥＮＥ６为介导，转染到大鼠脑恶性胶质瘤细胞 Ｃ６株 中，经ＭＴＴ检测，软琼脂集落生长，吖啶橙染色，琼脂糖电泳等方法分析实验结果。结果反义ｃ－ｍｙｃ基因质粒 ＤＮＡ具有抑制瘤细胞增殖的作用，并能引起瘤细胞凋亡；脂质体ＦｕＧＥＮＥ６有助于提高质粒ＤＮＡ的转染效率。结论 反义ｃ－ｍｙｃ基因质粒可抑制ｃ－ｍｙｃ基因的过度表达，促使肿瘤细胞凋亡，达到治疗脑神经胶质细胞瘤的目的。
Objective To investigate the effect of antisense c-myc plasmid on proliferation and apoptosis of C6 glioma cells. Methods Plasmid pCEP4ASCMR, which can express rat antisense c-myc gene in eukaryotic cells, was transfected into C6 glioma C6 cells mediated by liposome FuGENE6. MTT assay was used to detect the proliferation of soft agar colonies Growth, acridine orange staining, agarose electrophoresis and other methods analysis of experimental results. Results Plasmid DNA of antisense c-myc gene could inhibit tumor cell proliferation and induce apoptosis of tumor cells. Lipofectamine FuGENE6 could enhance the transfection efficiency of plasmid DNA. Conclusion Antisense c-myc gene plasmid can inhibit the overexpression of c-myc gene and promote the apoptosis of tumor cells, so as to achieve the goal of treating brain glioma.