论文部分内容阅读
目的 :探讨N 乙酰化转移酶 1(NAT1)基因多态性与肝癌遗传易感性的关系。方法 :应用PCR RFLP技术检测 96例肝癌患者和 173例对照的NAT1基因型 ,并分析NAT1与环境危险因素的交互作用。结果 :病例组等位基因NAT1 3,NAT1 4 ,NAT1 10和NAT1 14B的频率分别是 2 0 .3%、5 0 .5 %、2 4 .0 %和 5 .2 % ,基因型NAT1 3/ 3、NAT1 3/ 4、NAT1 3/ 10、NAT1 3/ 14B、NAT1 4 / 4、NAT1 4 / 10、NAT1 4 / 14B、NAT1 10 / 10和NAT1 10 / 14B的频率分别是 4 .2 %、2 5 .0 %、3.1%、4 .2 %、31.3%、10 .4 %、3.1%、14 .7和 4 .2 % ,与对照组相比差异均无统计学意义。病例组NAT1快型和慢型的频率分别是 32 .3%和 6 7.7% ,与对照组相比不存在统计学差异。NAT1 10与职业暴露存在交互作用 ,OR值为 3.4 0 (95 %CI:1.0 3～ 11.2 2 ) ,与其它环境因素不存在交互作用。结论 :NAT1基因多态性与肝癌不存在关联 ,NAT1 10与职业暴露存在交互作用
Objective: To investigate the relationship between N-acetyltransferase 1 (NAT1) gene polymorphism and genetic susceptibility to hepatocellular carcinoma. Methods: The genotypes of NAT1 in 96 HCC patients and 173 controls were detected by PCR RFLP. The interaction between NAT1 and environmental risk factors was analyzed. Results: The frequencies of alleles NAT1 3, NAT1 4, NAT1 10 and NAT1 14B in case group were 20.3%, 50.5%, 24.0% and 5.2% respectively. The genotype NAT1 3 / 3. The frequencies of NAT1 3/4, NAT1 3/10, NAT1 3 / 14B, NAT1 4/4, NAT1 4/10, NAT1 4 / 14B, NAT1 10/10 and NAT1 10 / 14B are respectively 4.2% There was no significant difference compared with the control group when they were 2.5%, 3.1%, 4.2%, 31.3%, 10.4%, 3.1%, 14.7% and 4.2% respectively. The fast and slow frequency of NAT1 in case group were 32.3% and 6 7.7%, respectively, which were not significantly different from the control group. There was an interaction between NAT1 10 and occupational exposure with an OR of 3.4 0 (95% CI: 1.0 3 to 11.2 2), which did not interact with other environmental factors. CONCLUSION: There is no association between NAT1 gene polymorphism and hepatocellular carcinoma, and NAT1 10 interacts with occupational exposure