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目的 :进一步了解端粒酶活性和端粒酶 RNA组分 (h TR)与喉癌发生、发展的关系 ,并建立喉癌的早期诊断标准。方法 :采用端粒酶重复扩增法和原位杂交法对正常组织、声带息肉、癌前病变和喉癌进行 h TR和端粒酶活性分析。结果 :1所有组织均表达不同水平的 h TR,h TR阳性率随着喉癌的临床分期逐渐增加 ,但无统计学差异。 2 39例 (78% )的喉癌组织表达端粒酶活性 ,声带息肉仅 1例 (8.3% )表达 ,不同分化的喉癌组织端粒酶活性有差异。结论 :端粒酶活化是喉癌发生的早期事件 ,检测端粒酶活性可判定临床喉部肿瘤的良恶性。但 h TR与端粒酶表达不一致 ,不能作为预测端粒酶活性的指标。
OBJECTIVE: To further understand the relationship between telomerase activity, telomerase RNA component (hTR) and occurrence and development of laryngeal carcinoma, and to establish an early diagnostic criteria for laryngeal cancer. Methods: hTR and telomerase activity of normal tissues, vocal cord polyps, precancerous lesions and laryngeal carcinoma were analyzed by repeated amplification of telomerase and in situ hybridization. Results: 1 All tissues expressed different levels of hTR, the positive rate of TR increased with the clinical stage of laryngeal cancer, but no statistical difference. The telomerase activity was detected in 39 cases (78%) of the laryngeal squamous cell carcinoma tissues and only 1 case (8.3%) of the vocal cord polyps were expressed. The telomerase activity in laryngeal squamous cell carcinoma tissues differed significantly. Conclusion: Telomerase activation is an early event in laryngeal carcinoma. The detection of telomerase activity can be used to judge the malignancy of clinical laryngeal carcinoma. However, h TR is not consistent with telomerase expression and can not be used as an indicator of telomerase activity.