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In order to understand the microtubule change of monocotyls stem-tip during mitosis,the arrangement,transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome,indirect immunofluorescence,DAPI staining and fluorescence microscopy.The results showed that nucleolus was intact when the cortical microtubules formed;cortical microtubules were changed into phramoplast microtubule bands at mitosis prophase.When phramoplast microtubule came into being,nuclear membrane was ruptured and chromosome was arranged at the position of cell plate;subsequently,phramoplast microtubules were changed into phragmoplast microtubules,phramoplast microtubules were shortening and microtubules on the sides of cell plate were increasing gradually,during this course sister chromatid was separated by microtubules at cell plate and tract to the two poles,forming phragmoplast microtubules.Then the nucleolus of two daughter cells formed and separated in the end with the increase of cells numbers.Therefore,cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.
In order to understand the microtubule change of monocotyls stem-tip during mitosis, the arrangement, transformation of microtubule array and its relation with chromosome movement during mitosis were studied with freezing microtome, indirect immunofluorescence, DAPI staining and fluorescence microscopy. The results showed that nucleolus was intact when the cortical microtubules formed; cortical microtubules were changed into phramoplast microtubule bands at mitosis prophase .When phramoplast microtubule came into being, nuclear membrane was ruptured and chromosome was arranged at the position of cell plate; subsequently, phramoplast microtubules were changed into phragmoplast microtubules, phramoplast microtubules were shortening and microtubules on the sides of cell plates were increased by, during this course sister chromatid was separated by microtubules at cell plate and tract to the two poles, forming phragmoplast microtubules. separate d in the end with the increase of cells numbers.Therefore, cell division orientation could be judged from the arrangement of cell microtubules in different periods in order to understand its growth status.