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目的建立一种新的适用于治疗重症急性胰腺炎(SAP)的猪模型,为SAP肠内给药提供实验和理论依据。方法8只太湖梅山猪随机分成SAP组和对照组,每组4只。两组均在开腹直视下置入鼻空肠管。SAP组通过胰管内注射5%牛黄胆酸钠建立SAP模型,对照组仅行胰管插管不注射任何药物。比较两组动物一般情况、血清淀粉酶、CT评分及胰腺病理形态学改变,验证鼻空肠管置入及SAP模型诱导是否成功。结果同对照组比较,SAP组血清淀粉酶的总体均数明显升高,差异有统计学意义(P<0.05);CT、病理评分均进一步证明了鼻空肠管置入及SAP模型诱导成功。结论在猪的SAP模型上成功置入鼻空肠营养管,为研究肠内给药在胰腺炎中的应用建立了一种简单、无创、可重复性的动物模型。
Objective To establish a new pig model for the treatment of severe acute pancreatitis (SAP) and provide experimental and theoretical basis for the enteral administration of SAP. Methods Eight Taihu Meishan pigs were randomly divided into SAP group and control group with 4 rats in each group. Two groups were placed nasal jejunal tube under open vision. In the SAP group, the SAP model was established by injecting 5% sodium taurocholate into the pancreatic duct. In the control group, only the pancreatic duct was intubated and no drug was injected. The general condition, serum amylase, CT score and the pathological changes of pancreas were compared between the two groups to verify the nasal jejunal tube placement and whether the SAP model was successfully induced. Results Compared with the control group, the overall average number of serum amylase in SAP group was significantly increased (P <0.05). CT and pathological score further proved the successful implantation of nasoguanjejunal tube and SAP model. Conclusions The nasal jejunal feeding tube was successfully inserted into the pig SAP model. A simple, non-invasive and reproducible animal model was established to study the application of enteral administration in pancreatitis.