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从贵州省大豆品种中随机抽取 1 44份进行脂肪氧化酶测定、豆奶加工试验和品质、农艺性状分析。结果发现了 3 3份脂肪氧化酶缺失品种 (其中 2份育成品种 ) ,占鉴定总数的 2 2 .9%,包括缺失 Lox2、Lox3和Lox2、3类型 ,以缺失 Lox3类型较多。缺失脂氧酶地方品种的蛋白质、脂肪含量较低 ,其氨基酸成分及比例与正常品种相近 ,而缺失的育成品种的蛋白质含量则高达 4 7%以上。利用强碱高温消除豆浆中的豆腥味后 ,正常品种豆浆中的氨基酸总量减少较多且部分氨基酸成分消失 ,缺失品种减少较少且氨基酸成份全部保留。Lox3缺失品种的脂氧酶活性与百粒重、株粒重负相关。脂氧酶缺失的育成品种有着较大的直接应用价值 ,而多数缺失的地方品种表现为重感病毒病和生育期太长的特点 ,需进行改良才能在生产上利用。
One hundred and seventy-four soybean samples were randomly selected from Guizhou Province for lipoxygenase assay, soybean milk processing test and quality and agronomic traits analysis. As a result, 33 lipoxygenase deficient varieties (2 of which were bred) were found, accounting for 22.2% of the total number of identified strains, including the deletion of Lox2, Lox3 and Lox2,3 types, and the lack of Lox3 types. The protein and fat content of local varieties lacking lipoperoxidase are lower, their amino acid composition and proportion are similar to those of normal varieties, but the protein content of the missing cultivars is as high as 47% or more. The use of strong base high temperature to eliminate the beany flavor of soy milk, the normal soybean milk in the total amino acid to reduce more and part of the disappearance of amino acid composition, loss of fewer varieties and all amino acid components retained. Lipoxygenase activity of Lox3-deficient cultivars was negatively correlated with 100-grain weight and grain weight. Lost varieties of lipoxygenase have a greater direct application value, while most of the missing local breeds showed the characteristics of heavy virus disease and the growth period is too long, need to be improved in order to use in production.