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本研究以红茄的子叶和下胚轴为外植体,研究其子叶和下胚轴在不同激素的浓度和组合下分化率及再生频率的变化。在诱导愈伤组织时,低浓度的NAA和高浓度的6-BA会导致下胚轴的诱导能力略低于子叶;IAA和ZT的浓度升高时,红茄下胚轴的分化率随之增加,红茄子叶的分化率随之降低。在IAA浓度为0.3 mg/L时,不定芽分化率达到最高;诱导愈伤组织的最佳培养基为MS+0.2 mg/L NAA+1.0 mg/L 6-BA;不定芽诱导最佳培养基为MS+0.3 mg/L IAA+2.0 mg/L 6-BA;不定芽生根最佳培养基为1/2MS。本研究建立的红茄再生体系,为红茄遗传转化研究奠定了基础。
In this study, the cotyledons and hypocotyls of Chinese cabbage were used as explants to study the changes of their cotyledons and hypocotyls under different hormonal concentrations and combinations. At the induction of callus, low concentration of NAA and high concentration of 6-BA resulted in slightly lower induction of hypocotyls than cotyledons; the differentiation rate of hypocotyl Increasing, red eggplant leaf differentiation rate decreased. The highest rate of adventitious bud differentiation was achieved when IAA concentration was 0.3 mg / L. The best culture medium for inducing callus was MS + 0.2 mg / L NAA + 1.0 mg / L 6-BA. MS + 0.3 mg / L IAA + 2.0 mg / L 6-BA. The optimal medium for rooting of adventitious buds was 1 / 2MS. The red tomato regeneration system established in this study laid the foundation for the genetic transformation of red tomato.