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免疫组织化学染色技术在实际运用中,由于切片厚薄的掌握不当、脱片及核对比染色欠佳等问题曾使许多研究者感到为难。我们在进行垂体腺瘤的免疫组化研究中也曾遇到上述问题,现将我们的作法及改进介绍如下。
In practice, the immunohistochemical staining technique has caused many researchers to feel embarrassed by the problems of inadequate mastery of slice thickness, poor desquamation and contrast staining. We have encountered the above problems in the study of immunohistochemistry of pituitary adenomas, now we introduce the practices and improvements are as follows.