初分离的人类多瘤病毒在细胞培养中繁殖极为缓慢,至少培养一个月后才可见到细胞病变,以致延误了对该病毒的临床诊断及流行病学调查。为克服这个缺点,国外学者已使用~(32)P标记的BKV探针在器官移植病人标本中检测该病毒的存在,但~(32)P在国内的供应及保存较受限制,且有放射性污染。本试验比较了我校药学院的生物素产品Bio-11-dUTP及显色系统碱性磷 The initial isolation of human polyoma virus propagation in cell culture is extremely slow, at least one month after the culture can be seen cytopathic effect, which delays the clinical diagnosis of the virus and epidemiological investigations. To overcome this shortcoming, foreign scholars have used ~ (32) P-labeled BKV probe to detect the presence of the virus in organ transplanted patients, but the supply and storage of ~ (32) P in China is more limited and radioactive Pollution. This experiment compares the biotin product Bio-11-dUTP and the alkaline phosphatase