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目的探讨缺血再灌注损伤对大鼠腮腺导管细胞TGF-β1、Bcl-2表达的影响。方法 wistar大鼠经颈总动脉结扎造成腮腺缺血再灌注模型,应用免疫细胞化学及一般光镜技术,分别观察缺血0.5 h和l h,再灌注6 h(早期)、12 h(中期)和24 h(后期)腮腺导管上皮细胞的TGF-β1和Bcl-2的动态表达情况及一般形态学变化。结果 1.TGF-β1:缺血再灌注组较对照组TGF-β1表达明显提高(P<0.01),且随缺血时间的延长,TGF-β1的表达呈逐渐增高趋势;组间比较,缺血1 h比0.5 h组表达提高(P<0.01);再灌注各组间两两比较差异均无统计学意义(P>0.05)。2.Bcl-2:再灌注6 h组的表达较对照组明显增高(P<0.05),而再灌注12、24 h较对照组差异无统计学意义(P>0.05);组间比较,缺血时间0.5 h与1 h组、再灌注各组间的两两比较差异均无统计学意义(P>0.05)。3.形态学:与对照组正常形态比较,实验组在缺血再灌注早期开始出现腺泡细胞萎缩、坏死等形态学改变,中期明显,后期修复。导管上皮细胞变化较轻。结论缺血再灌注可明显提高大鼠腮腺导管细胞TGF-β1的表达;缺血时间越长,TGF-β1表达上调越明显;缺血再灌注使Bcl-2的表达在早期明显升高;而中晚期Bcl-2的表达无显著变化;缺血再灌注对TGF-β1和Bcl-2表达的影响可能与形态学变化有关。
Objective To investigate the effect of ischemia-reperfusion injury on the expression of TGF-β1 and Bcl-2 in parotid ductal cells of rats. Methods Wistar rats were subjected to common carotid artery ligation to induce parotid ischemia-reperfusion injury. Immunocytochemistry and general light microscopy were used to observe the effects of ischemia 0.5 h and 1 h, reperfusion 6 h (early), 12 h Dynamic expression of TGF-β1 and Bcl-2 in parotid ductal epithelium at 24 h (late stage) and its general morphological changes. The expression of TGF-β1 in ischemic-reperfusion group was significantly higher than that in control group (P <0.01), and the expression of TGF-β1 was gradually increased with the prolongation of ischemia time. Compared between groups, Compared with 0.5 h group, the expression of HIF-1α was increased in 1 h and 0.5 h groups (P <0.01). There was no significant difference in any two groups between reperfusion groups (P> 0.05). The expression of Bcl-2 in 6 h reperfusion group was significantly higher than that in control group (P <0.05), while there was no significant difference between 12 h and 24 h reperfusion group (P> 0.05) Blood 0.5 h and 1 h group, no significant difference between the two groups (P> 0.05). Morphology: Compared with the normal control group, morphological changes of acinar cells atrophied and necrotic began to appear in the experimental group at the early stage of ischemia-reperfusion, and were obvious in the middle and repaired in the later period. Catheter epithelial cells change less. Conclusion Ischemia-reperfusion can significantly increase the expression of TGF-β1 in the parotid ductal cells of rats; the longer the ischemia is, the more obvious the up-regulation of TGF-β1 expression; the expression of Bcl-2 is significantly increased in the early stage of ischemia-reperfusion; The expression of Bcl-2 in the late stage had no significant change. The effect of ischemia-reperfusion on the expression of TGF-β1 and Bcl-2 may be related to the morphological changes.