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目的:研究小檗碱(BBR)对糖尿病肾病(DN)大鼠肾组织病理变化与骨形成蛋白-7(BMP-7)蛋白及mRNA表达的影响,探讨BBR对DN大鼠早期肾脏损伤的作用及其可能机制。方法:以链脲佐菌素(STZ)复制早期DN大鼠模型,动物分为正常对照组、模型组、3个BBR(50,100,200 mg.kg-1)治疗组及依那普利(1 mg.kg-1)治疗组,灌胃给药,qd,5周后检测各组大鼠尿量、左肾重、肾重/体重比值;生化指标检测空腹血糖(FBG)、尿素氮(BUN)、血肌酐(Scr)、胰岛素(Ins)、24 h尿蛋白量(Upro)及24 h尿微量白蛋白(Um-Alb);光镜和电镜观察肾组织形态学和超微结构的改变;免疫组化技术和逆转录聚合酶链反应(RT-PCR)检测肾组织BMP-7蛋白和mRNA表达。结果:与模型组比较,BBR各治疗组DN大鼠FBG,BUN,Scr,Upro及UmAlb水平降低,Ins升高;肾组织形态学异常和超微结构病变改善;BMP-7蛋白和mRNA表达增多。结论:BBR可显著升高早期DN大鼠肾组织BMP-7蛋白及mRNA的表达,改善早期DN大鼠肾功能病变,延缓DN发展。
AIM: To investigate the effect of berberine (BBR) on the pathological changes of renal tissue and the expression of BMP-7 protein and mRNA in diabetic nephropathy (DN) rats and to explore the effect of BBR on early renal injury in DN rats And its possible mechanism. Methods: The early DN model was established by streptozotocin (STZ). The animals were divided into normal control group, model group, three BBR groups (50,100,200 mg.kg-1) and enalapril (1 mg. kg-1). The rats in each group were given gavage qd for 5 weeks. Urine volume, left kidney weight and kidney weight / body weight ratio of rats in each group were measured. Fasting blood glucose (FBG), blood urea nitrogen (BUN) The levels of Scr, Ins, 24 hours urinary protein and 24 hours urinary albumin (Um-Alb) were measured. The morphological and ultrastructural changes of renal tissues were observed under light microscope and electron microscope. The expression of BMP-7 protein and mRNA in renal tissues was detected by RT-PCR and reverse transcription-polymerase chain reaction (RT-PCR). Results: Compared with the model group, the levels of FBG, BUN, Scr, Upro and UmAlb in DN rats in each BBR treatment group were decreased and Ins was increased; the morphological changes of renal tissue and the ultrastructure lesions were improved; the expressions of BMP-7 protein and mRNA were increased . Conclusion: BBR can significantly increase the expression of BMP-7 protein and mRNA in the early stage of DN rats, improve the renal function of DN rats and delay the development of DN.