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采集住院肺炎患儿鼻咽部分泌物,≥2岁患儿采集痰标本,应用直接免疫荧光法检测呼吸道病毒,应用荧光定量PCR检测呼吸道标本中的肺炎支原体(MP)和沙眼衣原体(CT);应用ELISA测定血清中的肺炎衣原体(CP)特异性IgM。结果 574例住院肺炎患儿中呼吸道病毒感染185例(32.2%),呼吸道合胞病毒(RSV)感染151例(26.3%),副流感病毒(PIV)感染24例(4.1%),腺病毒(ADV)感染6例(1.0%),流感病毒(IFV)感染4例(0.7%)。呼吸道病毒感染多发生于<2岁的患儿,共148例(40.9%)。非典型病原体感染108例(18.8%),其中MP感染76例(13.2%),CT感染21例(3.6%),CP感染11例(1.9%);≥2岁患儿MP感染率30.5%,<2岁患儿MP感染率为3.0%。结果表明,儿童呼吸道病毒与非典型病原体感染以RSV和MP多见,不同年龄段患儿各种病原体感染率有很大差异。
Nasopharyngeal secretions from hospitalized children with pneumonia were collected, and sputum samples were collected from children ≥2 years old. Respiratory viruses were detected by direct immunofluorescence. Mycoplasma pneumoniae (MP) and Chlamydia trachomatis (CT) were detected by real-time PCR. Serum Chlamydia pneumoniae (CP) -specific IgM was measured by ELISA. Results Among 574 hospitalized children with pneumonia, 185 (32.2%) were respiratory viruses, 151 (26.3%) were respiratory syncytial virus (RSV), 24 (4.1%) were parainfluenza virus (PIV) ADV infection 6 cases (1.0%), influenza virus (IFV) infection in 4 cases (0.7%). Respiratory virus infections occurred in children <2 years old, a total of 148 cases (40.9%). Among them, 108 cases were infected with atypical pathogens (18.8%), of which MP was infected in 76 cases (13.2%), CT infection in 21 cases (3.6%) and CP infection in 11 cases (1.9% MP infection rate in children <2 years old was 3.0%. The results showed that children with respiratory viruses and atypical pathogens RSV and MP more common infection, children of different ages have a very different infection rate of various pathogens.