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植物名称:南洋楹(Albizzia falcata)。材料类别:10~15日龄无菌苗。培养条件;基本培养基MS。(1)MS(不加激素)作培养无菌苗之用。诱导愈伤组织培养基为(2)MS+NAA2.0mg/L(单位下同)+BA0.5;(3)MS+IAA2.0+BA0.5;(4)MS+2,4-D1.0+KT0.5;(5)2/3MS+NAA0.2+BA0.5。(6)诱导芽分化培养基是MS+BA2.0~3.0+NAA0~0.1。(7)芽增殖培养基是MS+BA0.5~3.0+NAA0~2.0。(8)诱导生根培养基是1/2MS或MS+IBA0~3.0。各种培养基均用琼脂固化,pH5.8,光照强度2000
Plant name: Albizzia falcata. Material category: 10-15 days old sterile seedlings. Culture conditions; basic medium MS. (1) MS (without hormone) for the cultivation of sterile seedlings. The callus induction medium was (2) MS + NAA 2.0mg / L (unit same below) + BA0.5; (3) MS + IAA2.0 + BA0.5; .0 + KT0.5; (5) 2 / 3MS + NAA0.2 + BA0.5. (6) Induction bud differentiation medium is MS + BA2.0 ~ 3.0 + NAA0 ~ 0.1. (7) Bud proliferation medium is MS + BA0.5 ~ 3.0 + NAA0 ~ 2.0. (8) Induced rooting medium is 1 / 2MS or MS + IBA0 ~ 3.0. Various media were hardened with agar, pH5.8, light intensity 2000