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刺参养殖作为北方环渤海地区新兴的经济产业,池塘养殖是其主要的养殖模式,而传统养殖模式中其生长环境易受外界影响,池塘底泥和刺参消化道中菌群的变化易引起刺参病害的发生,本文旨在通过研究不同季节刺参肠道及刺参养殖池底泥中菌系多样性及变化为深入了解刺参池塘养殖系统微生物的结构与功能提供参考。运用纯培养方法和16SrRNA基因序列分析,在1年中分4次取样,从刺参肠道和刺参养殖池底泥中分离出共151株海洋菌株,其中肠道菌72株,养殖池塘底泥79株,发现新菌种5株,拮抗菌株1株,通过构建系统发育树对刺参肠道及养殖环境底泥多样性进行研究。结果显示:分离的菌株分属海王星菌属(Neptuniibacter)、假交替单胞菌属(Pseudoalteromonas)、迪茨氏菌属(Dietzia)、铁单胞菌属(Ferrimonas)、需盐杆菌属(Salegentibacter)等31个属;通过TCBS培养基筛选后发现弧菌属(Vibrio)在刺参肠道中有32株,底泥中28株,是刺参肠道和底泥环境中的第一大优势菌属,其数量随着时间变化呈上升趋势。
As a new economic industry in the northern Bohai Rim region, pond culture is the main breeding mode, while the growth environment in the traditional culture mode is susceptible to external influences. The changes in the pond sediment and the digestive tract in the sea cucumber are likely to cause thorns This paper aims to provide a reference for further understanding of the structure and function of microorganisms in the culture system of sea cucumber prickly heat by studying the diversity and changes of bacterial strains in the sediments of the sea cucumbers and sea cucumbers in different seasons. Pure culture method and 16S rRNA gene sequence analysis were used to sample four times in one year. A total of 151 strains of marine bacteria were isolated from the sea cucumber gut and the sea cucumber, including 72 intestinal bacteria, 79 strains of mud, 5 strains of new strains were found and 1 strain of antagonistic strain was found. The diversity of sediment in the intestinal tract and culture environment of A. brassicicola was studied by constructing phylogenetic tree. The results showed that the isolates belonged to the genus Neptuniibacter, Pseudoalteromonas, Dietzia, Ferrimonas, Salegentibacter, And other 31 genera. After screening by TCBS medium, 32 strains of Vibrio in the intestinal tract of Stichopus japonicus and 28 strains of sediment were found, which is the first predominant species in the intestinal tract and sediment of Stichopus japonicus , The number of which shows an upward trend with time.