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根据文献报道的恶性疟原虫裂殖子顶端膜抗原IDNA序列的保守区，设计并合成两对20聚寡核苷酸作引物，对恶性疟原虫勐捧分离株DNA进行PCR扩增。扩增产物经BamHI和EcoRI双酶解后，克隆入具有相应末端的M13mp8和M13mp8载体，转化JPA101，生长于含X－gal的培基上，抽提无色噬菌斑DNA，以DNA序列测定仪进行DNA序列测定，并自动翻释成氨基酸序列。用双脱氧终止法测定部分DNA序列，并与DNA序列测定仪测定的序列进行比较。结果表明，扩增的序列长度为1773个碱基，编码591个氨基酸。与参照序列比较，有17个点突变，引起15个密码子的取代，其中，除1个密码子为同义取代外，其余密码子均为非同义取代，造成14个氨基酸的取代。点突变在序列中呈散在分布，但在氨基酸序列中第160－210位相对较集中。
According to the conserved region of IDNA sequence of P. falciparum merozoite apical membrane antigen reported in the literature, two pairs of 20-mer oligonucleotides were designed and synthesized to amplify the DNA of Plasmodium falciparum isolates. The amplified product was double digested with BamHI and EcoRI, cloned into M13mp8 and M13mp8 vectors with the corresponding ends, transformed into JPA101, grown on X-gal-containing culture medium, and extracted the chromosomal plaque DNA to determine the DNA sequence The instrument performs DNA sequencing and automatically interprets the amino acid sequence. The partial DNA sequence was determined by dideoxy termination method and compared with the sequence determined by DNA sequencer. The results showed that the amplified sequence length of 1773 bases, encoding 591 amino acids. Compared with the reference sequence, there are 17 point mutations, resulting in the substitution of 15 codons. Among them, all the other codons are replaced by nonsynonymous codons, resulting in the substitution of 14 amino acids. Point mutations are scattered throughout the sequence, but are relatively concentrated at amino acid positions 160-210.