AZF deletion in patients with azoospermia and Oligozoospermia

来源 :Asian Journal of Andrology | 被引量 : 0次 | 上传用户:lovezjx
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Objective: To investigate the relationship between AZF deletion and azoospermia/ oligozoospermia. Methods: Multiplex PCR were use to detect the deletions of 4 STSs located at the AZF region, namely sY86 at AZFa, sY127 at AZFb and sY254 and sY255 at AZFc. PCR-SSCP was used to check the sY254 point mutations in 84 Chinese patients with azoospermia or oligozoospermia. The expression pattern of DAZ gene in different tissues was studied using the RT-PCR method. Results: The concurrent deletions of sY254 and sY255 in AZFc subregion were found in 3 azoospermia patients. There were no sY254 point mutation in 48 patients studied. DAZ gene was only expressed in the testicular tissue. Conclusion: AZFc deletions may be associated with azoospermia. Objective: To investigate the relationship between AZF deletion and azoospermia / oligozoospermia. Methods: Multiplex PCR were used to detect the deletions of 4 STSs located at the AZF region, namely sY86 at AZFa, sY127 at AZFb and sY254 and sY255 at AZFc . PCR-SSCP was used to check the sY254 point mutations in 84 Chinese patients with azoospermia or oligozoospermia. The expression pattern of DAZ gene in different tissues was used using the RT-PCR method. Results: The concurrent deletions of sY254 and sY255 in AZFc There were no sY254 point mutation in 48 patients studied. DAZ gene was only expressed in the testicular tissue. Conclusion: AZFc deletions may be associated with azoospermia.
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