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以5个白菜杂交种和8个亲本为试验材料,利用白菜单粒干种子DNA快速提取方法,筛选出能够区分5个杂交种与双亲,并具有互补带型的SSR引物,建立适于SSR分子标记检测白菜种子品种纯度的反应体系。试验结果证明这套检测技术体系可用于白菜杂交种品种纯度室内快速鉴定,从而为SSR分子标记技术在白菜种子纯度鉴定中的应用和推广打下了坚实的基础。
Five cabbage hybrids and eight parents were used as materials to screen SSR primers that could distinguish five hybrids and their parents and have complementary bands, The reaction system for detecting the purity of cabbage seeds was marked. The test results show that the system can be used for the rapid identification of the purity of cabbage hybrids in laboratory and thus lay a solid foundation for the application and promotion of SSR molecular marker in the identification of the purity of cabbage seeds.