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本研究通过调查异源三倍体为父本的百合杂交后代倍性及其追踪染色体来源,来探讨异源三倍体的遗传规律。采用核型分析方法,对以雄性不育的二倍体亚洲百合GHC-01为母本,以Longiflorum×Asiatic系列的异源三倍体百合‘Ceb Dazzle’为父本杂交获得的30个杂交后代及其父母本进行了染色体数目的统计,同时利用45S rDNA荧光原位杂交技术,对父母本以及4个杂交后代进行了45S rDNA荧光原位杂交分析。核型分析结果表明,GHC-01和‘Ceb Dazzle’的染色体数目分别为24(2n=2x=24)和36(2n=3x=36),30个杂交后代中染色体数目为24、25、26、35、36的分别有13、10、4、2、1个基因型的杂交后代;荧光原位杂交结果表明,GHC-01和‘Ceb Dazzle’分别有11个和14个45S rDNA荧光原位杂交位点,4个所研究的杂交后代中均有不同数目的典型父本染色体。基于倍性统计和荧光原位杂交结果,对杂交后代的早期鉴定以及异源三倍体在育种中的作用进行了讨论。
In this study, we investigated the inheritance of allotriploid by investigating the ploidy of the offspring of heterosis triploidy and the tracing of chromosome origin. The karyotype analysis method was used to analyze the genetic relationships among 30 hybrid progenies obtained from the diploid Asian lily GHC-01 male sterile and the male parent Longiflorum × Asiatic crossed with triploid lily ’Ceb Dazzle’ And their parents were counted by chromosome number. At the same time, 45S rDNA fluorescence in situ hybridization was used to analyze the 45S rDNA in situ hybridization of parents and four hybrids. The results of karyotype analysis showed that the chromosome numbers of GHC-01 and ’Ceb Dazzle’ were 24 (2n = 2x = 24) and 36 (2n = 3x = 36) respectively. The chromosome numbers of 30 hybrids were 24, 25, , 35 and 36 had 13, 10, 4, 2 and 1 genotypes, respectively. Fluorescence in situ hybridization showed that there were 11 and 14 45S rDNA fluorescence in situ in GHC-01 and ’Ceb Dazzle’ There were different numbers of typical paternal chromosomes in the hybrids and the four hybrids studied. Based on the results of ploidy statistics and fluorescence in situ hybridization, the early identification of hybrid progeny and the role of allo-triploid in breeding were discussed.