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采用核磁共振氢谱(1H NMR)获取不同转基因抗虫棉棉籽及其非转基因对照棉籽的代谢物谱,并结合多维统计分析探查植物基因工程操作是否引发棉籽中代谢产物种类和数量的非预期改变。结果表明,不同棉籽的1H NMR谱图轮廓基本特征相似,均可分为氨基酸区、碳水化合物区及芳香区;当谱图按分区扩展后,多处峰信号在转基因与非转基因对照间有明显的不同。采用偏最小二乘法-判别分析(PLS-DA)可实现转基因抗虫棉棉籽与非转基因对照的代谢轮廓判别且分类的效果优于主成分分析(PCA)方法。转基因抗虫棉籽与其非转基因对照间的主要差异代谢物与初级氮代谢、三羧酸循环及脂肪酸代谢有关。
The metabolite spectra of different transgenic Bt cottonseeds and their non-transgenic control cottonseeds were obtained by1H NMR, and multidimensional statistical analysis was used to explore whether plant genetic engineering operations caused unintended changes in the types and amounts of metabolites in cottonseed . The results showed that the basic features of the 1H NMR spectra of different cottonseeds were similar and could be divided into amino acid region, carbohydrate region and aromatic region. When the spectrum was extended by region, the signals of multiple peaks were obvious between transgenic and non-transgenic control s difference. Partial least square method-discriminant analysis (PLS-DA) is more effective than principal component analysis (PCA) in discriminating and classifying the metabolic profiles of transgenic Bt cottonseed and non-transgenic controls. The major differences between the transgenic Bt cottonseed and its non-transgenic controls were metabolites associated with primary nitrogen metabolism, tricarboxylic acid cycling, and fatty acid metabolism.