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乙型肝炎时肝细胞内可能存在IgG样物质利用人抗乙型肝炎病毒核心抗原抗体(抗HBc)作第一抗体,兔抗人IgG作第二抗体的方法检查肝组织内HBcAg,往往有假阳性结果。为避免肝细胞内IgG样物质干扰,提高检查肝组织内HBc-Ag特异性,我们对这种方法进行了改良。 一、材料 肝组织标本系用本院传染科住院病人肝活检组织,经中性福尔马林固定,石蜡包埋切片。 人抗HBc来自1例抗核抗体(ANA)、类风湿
IgG-like substances may be present in hepatocytes in hepatitis B. Hepatitis HBcAg may be detected using human anti-hepatitis B virus core antigen antibody (anti-HBc) as primary antibody and rabbit anti-human IgG as secondary antibody. Positive result. In order to avoid the interference of IgG-like substances in liver cells and improve the specificity of HBc-Ag in liver tissue, we have improved this method. 1. Materials Liver tissue specimens were obtained from liver biopsies of inpatients in the Department of Infectious Diseases of our hospital. The specimens were fixed in neutral formalin and embedded in paraffin. Human anti-HBc from 1 case of anti-nuclear antibody (ANA), rheumatoid