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目的 研究地塞米松、苯巴比妥钠和利多卡因预处理的昆明种小鼠 P45 0 3A酶有无诱导作用及其作用机制 .方法 给昆明种小鼠腹腔注射常用量地塞米松、苯巴比妥钠或利多卡因 1wk后 ,观察 MEGX累积排泄量、P45 0 3A酶蛋白含量及其 m RNA表达的改变 .结果 与正常对照组相比 ,地塞米松组小鼠 MEGX累积排泄量增加 35 .0 % (P<0 .0 5 ) ,P45 0 3A酶蛋白含量增加 147.1% (P<0 .0 1) ;苯巴比妥钠小鼠 MEGX累积排泄量增加 2 3.4% (P<0 .0 5 ) ,酶蛋白含量增加 43.6 % (P<0 .0 1) ;各组间 P45 0 3A酶 m RNA表达无明显差别 .结论 地塞米松和苯巴比妥钠对昆明种小鼠肝 P45 03A酶有诱导作用 ,该作用位于转录后水平 ;利多卡因对昆明种小鼠肝 P45 0 3A酶无影响
Objective To study the effect of dexamethasone, phenobarbital sodium and lidocaine preconditioning on the P45 0 3A enzyme in mice and its mechanism of action.Methods Kunming mice were injected intraperitoneally with dexamethasone, benzene After 1 wk of sodium pentobarbital or lidocaine, the cumulative excretion of MEGX, the protein content of P45 0 3A and the expression of m RNA were observed.Results Compared with the normal control group, the cumulative excretion of MEGX in dexamethasone group increased (P <0. 05), the protein content of P45 0 3A enzyme increased by 147.1% (P <0.01), and the cumulative excretion of MEGX by phenobarbital sodium increased by 23.4% (P <0 05) 0.05), the content of enzyme protein increased by 43.6% (P <0.01), there was no significant difference in the expression of P45 (superscript 3A) 3 mRNA among all groups.Conclusion Dexamethasone and phenobarbital could inhibit the growth of Kunming mouse liver P45 03A enzyme has an induction effect, which is located at the post-transcriptional level; Lidocaine has no effect on the liver P45 0 3A enzyme in Kunming mice