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目的:建立测定复方甘草口服溶液中愈创甘油醚含量的方法。方法:选用Accu BounDⅡC18固相萃取小柱对样品进行纯化,采用Hypersil ODS(4．6 mm×250 mm,5μm)色谱柱,以0．05 mol·L-1磷酸二氢钾溶液-0．0025 mol·L-1庚烷磺酸钠水溶液-乙腈(20:20:4)为流动相,流速为1．0 ml·min-1,检测波长为220 nm。结果:线性范围为5-40μg·ml-1,r=0．999 9;回收率为101．0％,RSD为1．5％。结论:该法简便快速、结果准确可靠、可用于复方甘草口服溶液中愈创甘油醚的含量测定。
Objective: To establish a method for determination of guaifenesin in compound licorice oral solution. Methods: The sample was purified with Accu BounDIIC18 solid phase extraction cartridge using Hypersil ODS (4.6 mm×250 mm, 5 μm) column with 0.05 mol·L-1 potassium dihydrogen phosphate solution - 0.0025. The mol.L-1 sodium heptane sulfonate aqueous solution-acetonitrile (20:20:4) was used as the mobile phase, the flow rate was 1.0 ml·min-1, and the detection wavelength was 220 nm. Results: The linear range was 5-40 μg·ml-1, r=0.999 9; the recovery was 101.0% and the RSD was 1.5%. Conclusion: The method is simple and rapid, accurate and reliable, and can be used to determine the content of guaifenesin in compound licorice oral solution.