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用大鼠作体内急性实验观察各种致(促)癌物对肝、腺胃及直肠等组织鸟氨酸脱羧酶(ODC)活性的诱导作用。结果发现:注射亚硝氨类致癌物[二乙基亚硝胺(DENA)及二甲基亚硝胺(DMNA)]24/小时可使肝中ODC活性分别升高12倍和3倍。促癌物苯巴比妥和巴豆油分别诱导4.5小时后,肝中ODC活性分别上升8.5倍和54倍;甲基胆蒽诱导24/小时后,肝中此酶活性上升6倍。分别灌入胃癌致癌物N—甲基—N—硝基—N—亚硝基胍(MNNG)或4—硝基—1—氧化喹啉(4—NQO)24小时后,大鼠腺胃粘膜ODC活性分别升高34倍和47倍。以促癌物脱氧胆酸钠保留灌肠3.5小时后,结肠和直肠粘膜ODC活性可诱导增高38倍。ODC与细胞增生及癌变关系密切,用致(促)癌物对ODC的诱导方法比较特异,可靠和迅速,因此可以考虑作为对肝、腺胃及结肠等消化道致(促)癌物检测筛选的生化指标。
Rats were used for acute experiments in vivo to observe the induction of ornithine decarboxylase (ODC) activity in liver, glandular stomach, and rectum by various chemo-promoting cancers. The results showed that nitrosamine carcinogens [DENA] and dimethylnitrosamine (DMNA) 24/hour could increase the ODC activity in the liver by 12 and 3 times, respectively. After 4.5 hours of induction of phenobarbital and croton oil, respectively, the ODC activity in the liver increased by 8.5-fold and 54-fold, respectively; after 24 hours of methylcholanthrene induction, the enzyme activity in liver increased by 6 times. After gastric cancer carcinogen N-methyl-N-nitro-N-nitrosoguanidine (MNNG) or 4-nitro-1-oxoquinoline (4-NQO) was injected into the stomach, the glandular and gastric mucosa of rats ODC activity increased 34-fold and 47-fold, respectively. After 3.5 hours of retention of enema with the sodium carbochodate, the cancer promoter, the mucosal and rectal mucosal ODC activity was induced to increase 38-fold. ODC is closely related to cell proliferation and canceration. The induction method of ODC induced by (promoting) cancer is specific, reliable, and rapid, so it can be considered as a screening test for liver cancer, adeno-gastric and colonic digestive tract. Biochemical indicators.