论文部分内容阅读
对浓缩铀２３５Ｕ内照射人淋巴细胞白血病细胞株Ｍｏｌｔ－４细胞和巨噬细胞株Ａｎａ－１细胞诱发的细胞凋亡及其防护因子进行了研究。实验中估算了２３５Ｕ在不同阶段培养细胞中的辐射累积吸收剂量。通过透射电镜的形态观察，发现Ｍｏｌｔ－４和Ａｎａ－１两株免疫细胞在受２３５Ｕ内照射作用下，可诱发核断裂，核染质边聚，以及呈现膜包裹着的凋亡小体形成为特征的细胞凋亡。而蛋白质合成抑制剂ＣＨＸ和ＲＮＡ合成抑制剂ＡｃｔＤ可显著抑制由核素２３５Ｕ诱发免疫细胞ＤＮＡ链断裂的作用，从而呈现出明显的对２３５Ｕ内照射诱发免疫细胞凋亡的防护作用。
Apoptosis and protective factors induced by enriched uranium 235U irradiated human lymphoblastic leukemia cell line Molt-4 and macrophage cell line Ana-1 were studied. In the experiment, we estimated the accumulated absorbed dose of 235U in cultured cells at different stages. Morphological observation by transmission electron microscopy revealed that Molt-4 and Ana-1 immune cells could induce nuclear breaks, nuclear chromatin agglomeration, and apoptotic bodies that were characterized by membrane-encapsulation under the irradiation of 235 U Apoptosis. However, CHX, an inhibitor of protein synthesis, and ActD, an inhibitor of RNA synthesis, significantly inhibited the DNA strand breaks induced by radionuclide 235U and showed a protective effect on apoptosis of immune cells induced by 235U irradiation.