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MID1编码R-R型的MYB转录因子,对不同的非生物胁迫均有响应,特别是在水稻(Oryza sativa)生殖期会受到干旱胁迫的诱导,进而在一定程度上可以保持花粉的育性并稳定水稻产量。为进一步研究水稻MID1对非生物胁迫的响应网络,利用酵母双杂交系统筛选出与其互作的蛋白因子OsMIP1,并利用双分子荧光互补系统在本氏烟草(Nicotiana benthamiana)细胞中得到验证。结果表明,OsMIP1编码1个预测含有ENTH/ANTH/VHS结构域的跨膜转运蛋白。OsMIP1在根、茎、叶、小穗和胚乳中均有表达。干旱胁迫下,OsMIP1在叶片和生殖器官中表达,特别是在减数分裂后的小花中表达显著上调。这些结果暗示,OsMIP1在花器官抵抗干旱胁迫中起一定的作用。在水稻营养生长阶段,OsMIP1表达还受到包括Na Cl和甘露醇在内的其它非生物胁迫的影响,暗示其可能在其它非生物胁迫调节中也具有一定的作用。植物中关于编码ENTH/ANTH/VHS结构域蛋白的研究很少。通过对MIP1亚家族进化关系进行分析,结果表明,在被子植物中,MIP1可分为6大类,这6大类分别来自被子植物祖先中原本就存在的6个拷贝,在被子植物的进化过程中又经历了多次基因重复和拷贝丢失等事件。MIP1家族成员广泛分布于被子植物中并可能具有抗胁迫等功能。
The MY1 transcription factor of RR type MID1 is responsive to different abiotic stresses, especially induced by drought stress in the reproductive stage of rice (Oryza sativa), which in turn can maintain pollen fertility and stabilize rice Yield. In order to further study the response network of rice MID1 to abiotic stress, the yeast two-hybrid system was used to screen out the protein factor OsMIP1 interacting with it and verified by Nicotiana benthamiana cells using bimolecular fluorescence complementation system. The results show that OsMIP1 encodes a transmembrane transporter that predicts an ENTH / ANTH / VHS domain. OsMIP1 was expressed in roots, stems, leaves, spikelets and endosperm. Under drought stress, OsMIP1 was expressed in leaves and reproductive organs, especially in florets after meiosis. These results suggest that OsMIP1 plays a role in the resistance of floral organs to drought stress. OsMIP1 expression was also impacted by other abiotic stresses, including NaCl and mannitol, during rice vegetative growth, suggesting that it may play a role in the regulation of other abiotic stresses. There is little research in plants on ENTH / ANTH / VHS domain proteins. By analyzing the evolutionary relationships of MIP1 subfamily, the results showed that in angiosperms, MIP1 can be divided into six categories, which are derived from 6 copies originally existed in the ancestors of angiosperms. In the process of the evolution of angiosperms In the repeated genetic duplication and copy loss and other events. MIP1 family members are widely distributed in angiosperms and may have anti-stress and other functions.