论文部分内容阅读
建立高效液相色谱法测定环维黄杨星D纳米乳中药物含量。通过有机溶剂法破乳,采用高效液相色谱法在205nm波长处测定峰面积,测定环维黄杨星D纳米乳中环维黄杨星D的含量。环维黄杨星D在0.0501—0.5010mg m L-1浓度范围内与峰面积呈良好的线性关系;回归方程为:A=520.83C+0.8515,r=0.9992(n=5);平均回收率为99.31%、RSD=0.89%;日内及日间精密度的RSD分别为0.61%和0.94%(n=5)。建立的高效液相色谱法准确度高、专属性好,可用于环维黄杨星D纳米乳中环维黄杨星D的含量测定。
To establish a HPLC method for the determination of drug content in ring Waxing D nanoemulsion. By organic solvent demulsification, high performance liquid chromatography peak area was measured at 205nm wavelength, determination of ring Weiyang Yang D nanoemulsion ring in the content of vimentin D. Cyclovirobuxine D had a good linear relationship with the peak area in the range of 0.0501-0.5010mg m L-1. The regression equation was: A = 520.83C + 0.8515, r = 0.9992 (n = 5) 99.31%, RSD = 0.89%. The intra-day and inter-day RSD were 0.61% and 0.94%, respectively (n = 5). The established HPLC method has the advantages of high accuracy and good specificity, and can be used for determining the content of vimentin D in bovine lumen D nanoemulsion.