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本文建立了用高效液相色谱法测定人体内炎痛喜康血药浓度的分析方法,以YWG-C_(18)化学键合相(10μm)为固定相,以0.2 M醋酸铵—甲醇(1:1 Ⅴ/Ⅴ)为流动相,非那西汀为内标,使用国产HPLC仪,UV 254 nm检测器,得出检出限为5 ng,最低检测浓度为0.05μg/ml血清,线性范围为0.1～10μg/ml,方法回收率为99.28%。本法简便、重现性好、专一性强。健康志愿者口服炎痛喜康20 mg一片后,用本法测定得到的药动学参数与文献报道值接近。
In this paper, a method for the determination of the concentration of Yikongxikang in human body by high performance liquid chromatography has been established. The YWG-C 18 chemically bonded phase (10μm) was used as the stationary phase and 0.2 M ammonium acetate-methanol (1: 1 V / V) as the mobile phase, phenacetin as the internal standard, using a domestic HPLC instrument, UV 254 nm detector, the detection limit was 5 ng, the lowest detection concentration of 0.05μg / ml serum, the linear range 0.1 ~ 10μg / ml, the recovery rate was 99.28%. This Law is simple, reproducible, and specific. After oral administration of 20 mg of piroxicam to healthy volunteers, the pharmacokinetic parameters measured by this method are close to those reported in the literature.