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采用胰酶冷消化法对胚胎鼠胃粘膜上皮细胞进行了培养，细胞培养于含２０％的胎牛血清的ＤＭＥＭ／Ｆ１２的培养液中，种植２４ｈ后开始生长，３ｄ长成片状，相差显微镜下观察９０％的细胞具有上皮细胞特征。免疫细胞化学显示：（ａ）９０％的细胞上皮角蛋白染色阳性；（ｂ）９０％的上皮细胞ＰＡＳ染色阳性；（ｃ）２０％的上皮细胞琥珀酸脱氢酶（ＳＤＨ）染色阳性。透射电镜可见微绒毛、连接复合物（紧密连接，桥粒）、丰富的糖原、线粒体。放射自显影结果表明：上皮细胞具有合成ＤＮＡ的能力，而且在培养２ｄ时增殖能力最强。本实验为研究胃粘膜的功能提供了较好的方法。
The gastric mucosal epithelial cells of embryonic mice were cultured by trypsin cold digestion. The cells were cultured in DMEM / F12 medium containing 20% fetal bovine serum. After 24h cultivation, the cells began to grow and grew into the shape of flakes. Phase contrast microscopy Under the observation of 90% of the cells have epithelial cell features. Immunocytochemistry showed: (a) 90% of epithelial keratin staining; (b) 90% epithelial PAS staining positive; (c) 20% of epithelial cells were positive for succinate dehydrogenase (SDH) staining. Transmission electron microscopy shows microvilli, connective complexes (tight junctions, desmosomes), abundant glycogen and mitochondria. The results of autoradiography showed that the epithelial cells had the ability to synthesize DNA and had the strongest proliferation ability on the 2nd day. This experiment provides a better method for studying the function of gastric mucosa.