论文部分内容阅读
癌症病人在尿中排放比常人高得多的叶啉和嘧啶，这些改性核耷是RNA的主要组成。由于其增加的排放与癌变的RNA周转有关，已被建议和研究作肿瘤标记物。文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。两种方法所得数据一致。用此方法建立了正常人尿中核苷的排放水平，并测定了34个癌症病人尿中核苷浓度，观察到改性核着浓度明显的增高现象。结果袭明，两个方法均适于大量尿样的核苷与癌症关系的临床研究。
Cancer patients in the urine than much higher emissions of phytohormones and pyrimidines, these modified nucleotides is the main component of RNA. Tumor markers have been proposed and studied as their increased emissions are associated with cancerous RNA turnover. The article gives the determination of urinary nucleosides by reversed-phase high performance liquid chromatography and capillary electrophoresis. The data obtained by the two methods are consistent. Using this method, normal human urinary nucleoside levels were established and the concentration of urinary nucleosides in 34 cancer patients was measured. The marked increase in the concentration of modified nuclei was observed. The results confirm that both methods are suitable for clinical studies of the association of large numbers of urine with nucleosides.