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目的研究多次氯胺酮给药对大鼠海马CA1区突触长时程增强(LTP)的影响。方法 30只SD大鼠,年龄35-45 d,随机分为Con1组、Con2组、Ket1组、Ket2组及Ket3组(n=6)。Con1组单次腹腔注射生理盐水2 ml,Con2组每3日腹腔注射生理盐水2ml一次,共5次,Ket1组单次腹腔注射氯胺酮70 mg·kg-1(生理盐水稀释至2 ml),Ket2组每3日腹腔注射氯胺酮70 mg·kg-1(生理盐水稀释至2ml)共3次,Ket3组每3日腹腔注射氯胺酮70 mg·kg-1(生理盐水稀释至2 ml)共5次。末次给药7 d后,制作海马脑片,应用大鼠海马脑片细胞外记录技术检测海马CA1区LTP和强直刺激后群体峰电位振幅(PSA)的变化。结果与Con1组、Con2组比较,Ket1组PSA与LTP诱发率差异无统计学意义 (P>0．05),Ket3组PSA与LTP诱发率降低(P<0．01)。结论多次氯胺酮给药可降低大鼠海马CA1 区突触的可塑性。
Objective To investigate the effect of multiple ketamine administration on long-term potentiation (LTP) of hippocampal CA1 region in rats. Methods Thirty SD rats aged 35-45 days were randomly divided into Con1 group, Con2 group, Ket1 group, Ket2 group and Ket3 group (n = 6). The Con1 group received intraperitoneal injection of 2 ml of normal saline and the Con2 group received intraperitoneal injection of 2 ml of normal saline 2 times every 3 days. Ket1 group was intraperitoneally injected with ketamine 70 mg · kg-1 (diluted to 2 ml with normal saline), Ket2 The rats in group Ket3 were injected intraperitoneally with ketamine 70 mg · kg-1 (normal saline diluted to 2 ml) every three days for 3 times. Ket3 was injected intraperitoneally with ketamine 70 mg · kg-1 (normal saline diluted to 2 ml) every 5 days for 5 times. Seven days after the last administration, hippocampal slices were made and the hippocampal slices of rats were subjected to extracellular recording technique to detect the change of peak potential amplitude (PSA) after LTP in hippocampal CA1 region and tonic stimulation. Results Compared with Con1 and Con2 groups, there was no significant difference in the induction rates of PSA and LTP between Ket1 group and Ket3 group (P <0.01). Conclusions Administration of ketamine for many times can reduce synaptic plasticity in hippocampal CA1 area of rats.