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目的 探索CTLA4Ig基因在实验动物体内的表达以及对大鼠同种心脏移植后的免疫抑制作用。方法 利用腺病毒作载体 ,将CTLA4Ig基因导入同种心脏移植的受体鼠体内 ,以编码 β 半乳糖苷酶的有复制缺陷的腺病毒重组体 (Adex/LacZ)为对照 ,观察该基因在实验动物体内的表达以及对同种异体心脏移植后的免疫抑制作用。逆转录多聚酶链反应 (RT PCR)法检测CTLA4Ig基因在大鼠体内的表达 ,并用流式细胞仪来测定血中CTLA4Ig蛋白质的浓度变化。 结果 导入的CT LA4Ig基因能够在大鼠肝脏中表达 ,血中CTLA4Ig的浓度明显高于对照组 (P <0 .0 5 ) ,而且移植后心脏的存活时间显著长于对照组 (P <0 .0 1)。结论 CTLA4Ig基因能在大鼠体内良好表达并明显拮抗同种移植排斥反应
Objective To explore the expression of CTLA4Ig gene in experimental animals and the immunosuppressive effect of CTLA4Ig on rat heart allografts. Methods The adenovirus vector was used to transfer the CTLA4Ig gene into recipients of the same type of heart transplantation. The recombinant adenovirus carrying Aβ / LacZ encoding β-galactosidase was used as control. Expression in animals and immunosuppression after allograft heart transplantation. Reverse transcriptase polymerase chain reaction (RT PCR) was used to detect the expression of CTLA4Ig gene in rat. The concentration of CTLA4Ig protein in blood was measured by flow cytometry. Results CT LA4Ig gene was expressed in rat liver. CTLA4Ig level in blood was significantly higher than that in control group (P <0.05), and the survival time of heart after transplantation was significantly longer than that of control group (P <0. 0 1). Conclusion CTLA4Ig gene can be well expressed in rats and significantly antagonize allograft rejection